The Basilica of St. Bartholomew on the Island (Italian: Basilica di San Bartolomeo all'Isola, Latin: Basilica S. Bartholomaei in Insula) is a titular minor basilica, located in Rome, Italy. It was founded in 998 by Otto III, Holy Roman Emperor and contains the putative relics of St. Bartholomew the Apostle. It is located on Tiber Island, on the site of the former temple of Aesculapius, which had cleansed the island of its former ill-repute among the Romans and established its reputation as a hospital, continued under Christian auspices today.

Its cardinal priest has been Cardinal Blase Cupich since 19 November 2016.

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